Please fill in the content of application form accurately. Detailed information helps to identify the most appropriate conditions.
We recommend you to estimate cell size with a microscope and make sure that the fluorescent labeling is successful.
Please prepare a suitable control and buffer for your experiment.
Such as: (1) wild type control
(2) normal donor samples
(3) unstained sample – to define background fluorescence
(4) single-stained samples - for fluorescence compensation
(5) nonspecific binding control – to confirm antibodies specificity
Please adjust samples concentration to at least 2x10^5 cells/ml.
To avoid blocking, samples have to be pre-filtrated with mesh.