Experiment FAQ

  • Please fill in the content of application form accurately. Detailed information helps to identify the most appropriate conditions.

  • We recommend you to estimate cell size with a microscope and make sure that the fluorescent labeling is successful.

  • Please prepare a suitable control and buffer for your experiment.

    Such as: (1) wild type control

                      (2) normal donor samples

                      (3) unstained sample – to define background fluorescence

                      (4) single-stained samples - for fluorescence compensation

                      (5) nonspecific binding control – to confirm antibodies specificity

  • Please adjust samples concentration to at least 2x10^5 cells/ml.

  • To avoid blocking, samples have to be pre-filtrated with mesh. 

設施服務日曆 Service Calendar

線上申請表單 Application Form

設施諮詢 Consultation

營運經理: 曾于芳

設施電話: 02-2787-1013

聯絡信箱: bluegill@gate.sinica.edu.tw

設施位址: 中研院 植微所 R104室

 

Operation Manager: April Tseng

TEL: +886-2-2787-1013

Email: bluegill@gate.sinica.edu.tw

Address:  Room 104, Institute of Plant and Microbial Biology, Academia Sinica.